Journal: Investigative Ophthalmology & Visual Science

Article Title: Ferroptosis: A Novel Mechanism in Diabetic Keratopathy

doi: 10.1167/iovs.67.2.41

Figure Lengend Snippet: Increased Prussian blue iron staining in human diabetic donor corneal biopsy tissue. ( A ) Representative images of whole mount preparations of human corneal biopsy punches from 69-year-old nondiabetic (NDM) and 64-year-old diabetic (DM) human donors. These punches were stained with Prussian blue and eosin. Scale bar , 200 µm. ( B ) Representative corneal epithelial images used for the semiquantitative iron staining score analysis. Scale bar , 50 µm. ( C ) Increased Prussian blue iron staining in human diabetic donor corneal epithelium ( n = 5). All values are mean ± SD.

Article Snippet: HCEC were cultured in Corneal Epithelial Cell Basal Medium (PCS-700-030; ATCC) with Corneal Epithelial Cell Growth Kit (PCS-700-040; ATCC), following the manufacturer's recommended protocol.

Techniques: Staining

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ferroptosis: A Novel Mechanism in Diabetic Keratopathy

doi: 10.1167/iovs.67.2.41

Figure Lengend Snippet: Iron accumulation and TfR1 upregulation in STZ-diabetic (STZ-DM) mouse corneas. ( A ) Representative Prussian blue staining images showing iron staining in paraffin sections from STZ-DM mouse corneas. The arrow indicates the location of the iron staining in the cornea. ( B ) STZ-DM mice exhibit a significant reduction in corneal epithelial thickness compared with nondiabetic (NDM) controls ( n = 5). ( C ) Representative corneal flat mounts image (with epithelium side up) showing Prussian blue staining for iron in the corneas of NDM and STZ-DM mice. The arrows indicate the location of the iron staining in the cornea. ( D ) Increased iron staining scores in corneal flat mounts from STZ-DM mice ( n = 5). ( E , F ) Western blot showing increased TfR1 expression in STZ-DM mouse corneas ( n = 5). All values are mean ± SD.

Article Snippet: HCEC were cultured in Corneal Epithelial Cell Basal Medium (PCS-700-030; ATCC) with Corneal Epithelial Cell Growth Kit (PCS-700-040; ATCC), following the manufacturer's recommended protocol.

Techniques: Staining, Western Blot, Expressing

Journal: bioRxiv

Article Title: SenCat: Cataloging human cell senescence through multiomic profiling of multiple senescent primary cell types

doi: 10.64898/2026.02.05.703986

Figure Lengend Snippet: (A) Schematic of the experimental design for induction of systemic senescence with doxorubicin and sample collection across time points. (B) Cluster identities assigned to doxorubicin-treated lung samples profiled by snRNA-seq. (C) UMAP projection of lung samples colored by cluster identity. (D) UMAP projections of senescent cells in lung samples across time points. (E) Bar plot showing the number of senescent cells from lung per cluster and condition. (F,G) GSEA plots of gene set association scores for p53, EMT, NF-κB, Apoptosis, and Hypoxia hallmark pathways (F) and senescence signature lists SenePy, SeneSig, SenMayo, hUSI (G); in fibroblast, epithelial, and endothelial clusters on Day 6. (H) Schematic of the analysis pipeline applied to published aging lung snRNA-seq datasets. (I) UMAP projection of aging lung samples showing senescent cell distribution by age group. (J) Schematic of ligand-receptor inference analysis between senescent fibroblasts and non-senescent epithelial cells. (K) Chord diagram displaying ligand-receptor interactions between senescent fibroblasts (sender cells) and non-senescent epithelial cells (receiver cells) inferred through CellPhoneDB. (L) Dot plot showing expression of the specified ligands across fibroblast clusters at 23 months of age. (M) Schematic and GSEA plot explaining and displaying TGFβ signaling pathway increase during aging in non-senescent epithelial cells at 23 months versus 3 months. See also Figures S11-13 .

Article Snippet: HSAEC lung epithelial cells (ATCC, PCS-301-010) were cultured using an Airway Epithelial Cell Basal Medium plus Bronchial Epithelial Cell Growth Kit (ATCC).

Techniques: Expressing